Trying to make a MORE EFFICIENT RT-PCR!!

sexychicky! · #1 (**permalink**) 12-06-2006, 03:56 AM

I'm trying to make more efficient an RT-PCR.... i have my desired PCR product, i wish to get a better reaction..... the problem I think is that the primers are getting in dimers. I've heard about the use of detergents or DMSO in the reaction mix but i'm not sure about this. Does anyone knows a way to do it without change the Tm?

tuttu · #2 (**permalink**) 12-06-2006, 04:16 PM

hi,

As quoted elsewhere in this site, a hotstart will be a very simple and an yielding strategy!!.

cheers

sexychicky! · #3 (**permalink**) 12-07-2006, 06:19 AM

thanks tuttu!

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
protein is interesting ,modify it to make it more useful	zhangwenhui	Protein Science	0	11-06-2007 11:45 AM
How Eating Less Might Make You Live Longer	admin	General Science Questions and Layperson Board	1	07-26-2007 06:08 PM
How to Make a Stable Cell Line Using Suspension Culture?	oBWhat	Cell Biology and Cell Culture	0	06-19-2007 04:40 PM
a gene that make it possible to distinguish...	dee_dee A	DNA Techniques	1	04-19-2007 10:11 PM
How should I make LB solution?	Dionisia	Basic Lab Protocols and Techniques	1	02-02-2006 03:20 AM

Member Login

Trying to make a MORE EFFICIENT RT-PCR!!

Trying to make a MORE EFFICIENT RT-PCR!! - PCR - Polymerase Chain Reaction Forum

Trying to make a MORE EFFICIENT RT-PCR!! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.