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Trying to make a MORE EFFICIENT RT-PCR!!

Trying to make a MORE EFFICIENT RT-PCR!! - PCR - Polymerase Chain Reaction Forum

Trying to make a MORE EFFICIENT RT-PCR!! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-06-2006, 03:56 AM
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Thumbs up Trying to make a MORE EFFICIENT RT-PCR!!



I'm trying to make more efficient an RT-PCR.... i have my desired PCR product, i wish to get a better reaction..... the problem I think is that the primers are getting in dimers. I've heard about the use of detergents or DMSO in the reaction mix but i'm not sure about this. Does anyone knows a way to do it without change the Tm?
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Old 12-06-2006, 04:16 PM
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Default Re: Trying to make a MORE EFFICIENT RT-PCR!!

hi,

As quoted elsewhere in this site, a hotstart will be a very simple and an yielding strategy!!.

cheers
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Old 12-07-2006, 06:19 AM
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Default Re: Trying to make a MORE EFFICIENT RT-PCR!!

thanks tuttu!
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