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Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?

Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA? - PCR - Polymerase Chain Reaction Forum

Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-27-2009, 08:16 PM
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Default Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?



Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?
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Old 10-27-2009, 08:47 PM
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Default Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA

I believe the premise of your question is wrong. Did someone tell you this was a fact?
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Old 10-28-2009, 05:56 PM
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Default Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA

It doesn't. Amplification starts in the first cycle, although it usually takes at least 10 before detectable amounts of product are visible.

Bryan
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