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Curious Person

10-27-2009 08:16 PM

Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?

Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?

danfive

10-27-2009 08:47 PM

Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA

I believe the premise of your question is wrong. Did someone tell you this was a fact?

Warthaug

10-28-2009 05:56 PM

Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA

It doesn't. Amplification starts in the first cycle, although it usually takes at least 10 before detectable amounts of product are visible.

Bryan

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