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-   -   Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA? (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/71027-why-do-first-2-cycles-pcr-polymerase-chain-reaction-produce-0-target-dna.html)

Curious Person 10-27-2009 08:16 PM

Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?
 
Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA?

danfive 10-27-2009 08:47 PM

Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA
 
I believe the premise of your question is wrong. Did someone tell you this was a fact?

Warthaug 10-28-2009 05:56 PM

Re: Why do the first 2 cycles of PCR (Polymerase Chain reaction) produce 0 target DNA
 
It doesn't. Amplification starts in the first cycle, although it usually takes at least 10 before detectable amounts of product are visible.

Bryan


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