I need help with these simple questions:
1. PCR consists of 3 major steps: Dissociation, Annealing and synthesis at particular temperatures. What would happen if the temp. was either too high or too low at each of those steps.
2. What would be the consequence if ONE of the primers used had 4 mismatched bases in the middle?
3. We ran PCR for 30 cycles. What would be the consequence of running PCR 1 cycle less? 5 cycles less? 10 cycles less?
4. What would happen if we only used one primer (left or right) in a PCR reaction?
I generally know the answers, I just need to be sure and if I can get thorough and specific answers, that would be great. Thanks!