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Pcr - PCR - Polymerase Chain Reaction Forum

Pcr - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 10-21-2009, 06:24 AM
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Question Pcr



hi
Is that so.....
I did my pcr continuously , and got favourable result. but suddenly none of my pcr reaction is working .... i dont know why this happens . Every thing is same but the i m getting shears of DNA near the well instead of Band..
Anyone know......
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  #2  
Old 11-04-2009, 02:22 PM
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Default Re: Pcr

k u r geting a highy MW dna just chech out designg new primers
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Old 11-10-2009, 07:19 AM
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Smile Re: Pcr

Thanks for ur reply.
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Old 01-25-2010, 11:06 AM
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Default Re: Pcr

Quote:
Originally Posted by charumathi View Post
hi
Is that so.....
I did my pcr continuously , and got favourable result. but suddenly none of my pcr reaction is working .... i dont know why this happens . Every thing is same but the i m getting shears of DNA near the well instead of Band..
Anyone know......
Same problem here.
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  #5  
Old 01-25-2010, 07:13 PM
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Default Re: Pcr

DNase contamination or degraded primers
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  #6  
Old 01-28-2010, 09:12 AM
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Default Re: Pcr

Well, you say that everything is the same, it's clearly not. Something must have been changing. I would try to do everything over again with only new reagents all over.
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