thank you guys for all the great information. Aga..i already take note for everything you told for the last post.Thanks..i'll try to do the checklist again for those sample that i havent get any result..
selvam..all the best for you, thanks for the sharing..good for you that you solve your problem.
here come the other problem guys
hihihi.. after the PCR, and we load into the gel for electroforesis, i got band, but that band is not good enough (means it just a tiny, smear band) and below of the band got smear which is so light...the smear is more light rather than the band...ohh i hope you guys understand what i'm saying
for the information here some of my volume(ul) of the reagent i'm using
MgCl 3, buffer 5, dNTP 0.5, primers(2) 4each, taq 0.125, ddh20 8.175, DNA 0.2
I'm doing PCR-RFLP for IGS region..