Primer

[charumathi]

hi
I m new to this primer designing technique.
Can any one tell me what all the parameters(i.e Tm etc) to be noted / known for designing a primer.

Thanks ..

[charumathi]

Originally Posted by charumathi

hi
I m new to this primer designing technique.
Can any one tell me what all the parameters(i.e Tm etc) to be noted / known for designing a primer.

Thanks ..

"And i need to know how to insert a restriction site before and after the primers(forward and reverse)."

[jiajia1987]

Perhaps the following links will be helpful..

[Only registered users see links. ]

[Only registered users see links. ]

[Only registered users see links. ]

If you are still unclear after reading, just drop a few questions (that are a little more specific) here. =)

[charumathi]

hi
Thanks for ur reply , i will go thro and ask if i have any doubts.

[lala0453]

A very good post and certainly these are points really useful.

[ramnivas]

you can find charecterstic features of primers in any good book of molecular biology.
You can design the primers using gene runner sofware it will show the all features of a perticualr primer.