error prone pcr

shurgood · #1 09-20-2009, 02:48 AM

Does anyone have a protocol or know where I can find one? Thanks!

davidflora · #2 01-28-2013, 08:43 AM

[Only registered users see links. ] Error prone PCR is a method by which random mutants maybe inserted into any piece of DNA. The technique is based on the well founded PCR (polymerase chain reaction), which is a standard technique in many molecular biology laboratories. Normally the replication of DNA by the polymerase is extremely specific,The difference in error prone PCR is that the fidelity of the Taq DNA polymerase is modulated by alteration of the composition of the reaction buffer.

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error prone pcr

error prone pcr - PCR - Polymerase Chain Reaction Forum

error prone pcr - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.