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| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
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#1
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| successful.? The polymerase chain reaction (PCR) requires __________ and __________ to be successful. A. RNA nucleotides and RNA polymerase B. proteins and nucleotides C. taq and polymerase D. DNA nucleotides and DNA polymerase |
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ramnivas (09-07-2009)
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#2
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| I would say C based on the reagents I've used before. |
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#3
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| I would say D. taq and polymerase are the same thing, isn't it? |
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#4
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| Taq is a type of polymerase, yeah. I was on the fence with D too.. |
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#5
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| I would say D bcoz PCR requires dNTPs and Taq DNA polymerase both. |
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#6
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| A. RNA nucleotides and RNA polymerase - No. RNA must be reverse transcribed into cDNA before PCR can be done. B. proteins and nucleotides - Technically No. See d. C. taq and polymerase - No. Same thing, here. Taq DNA Polymerase. D. DNA nucleotides and DNA polymerase - Yes. dNTPs, nucleotides, are the building blocks. DNA polymerase, a protein (enzyme), is the engine needed to create the polymer chain. |
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#7
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| I would say no one. Because PCR requires 10 × amplification buffer,dNTP mixture ,2 primer,template DNA and Taq DNA polymerase . |
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#8
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| PCR reqiures DNTP's and Polymerase (i.e) D. Along with this Buffer is also required. |
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| chain , pcr , polymerase , reaction , requires |
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