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trisHcl or just Tris base

trisHcl or just Tris base - PCR - Polymerase Chain Reaction Forum

trisHcl or just Tris base - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 08-31-2009, 10:09 PM
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Default trisHcl or just Tris base



Hi everyone,
I would like to know what is the diference using TrisHCL or Tris (just like that) to prepare 1M TRIS solution. So what I heard is that you don't have to use a solution of HCL to get the pH that you want. If someone have a recipe using TRISHCL please let me know

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Old 09-01-2009, 01:44 AM
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Default Re: trisHcl or just Tris base

I think it really depends on what pH you really want...
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Old 09-01-2009, 04:22 PM
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Default Re: trisHcl or just Tris base

Hello,
I need pH 8 but I think you use less HCL to reach that pH..Right?
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Old 09-02-2009, 02:21 AM
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Default Re: trisHcl or just Tris base

vogmall.com
So wonderful website!
Do not miss it!
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Old 09-03-2009, 01:12 AM
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Default Re: trisHcl or just Tris base

Hi mcordero,

Yes, pH8 requires less HCL to reach that pH. Just calculate the total amount of water you need to add to the powder to get the concentration that you want first. Then, add half the amount of water to the powder and add HCL slowly and measure the pH until it reaches 8 before topping up the volume to the total amount of water you need to get the desired concentration.

I needed pH 7.4 once and it took a considerable amount of HCL.
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The Following User Says Thank You to jiajia1987 For This Useful Post:
mcordero (09-06-2009)
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