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IS there any different in using mili Q water instead of DEPC water for PCR?

IS there any different in using mili Q water instead of DEPC water for PCR? - PCR - Polymerase Chain Reaction Forum

IS there any different in using mili Q water instead of DEPC water for PCR? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 08-28-2009, 07:54 AM
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Default IS there any different in using mili Q water instead of DEPC water for PCR?



Hi,


I am currently trying to amplify a sample. But I noticed there were no amplification for my PCR. I used miliq water instead of DEPC water, will that affect my PCR reaction?
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Old 08-28-2009, 04:34 PM
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Default Re: IS there any different in using mili Q water instead of DEPC water for PCR?

No, it should not. In fact, milliQ water is the better recommended option for running PCR. DEPC-treated water afterall, contains small amounts of DEPC. Although they generally should not affect the PCR process, it's better to use milliQ water as it's filtered, UV-ed and deionized (if I'm not mistaken).
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