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#1
08-28-2009, 07:54 AM
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 IS there any different in using mili Q water instead of DEPC water for PCR? Hi, I am currently trying to amplify a sample. But I noticed there were no amplification for my PCR. I used miliq water instead of DEPC water, will that affect my PCR reaction?  |
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#2
08-28-2009, 04:34 PM
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| Re: IS there any different in using mili Q water instead of DEPC water for PCR? No, it should not. In fact, milliQ water is the better recommended option for running PCR. DEPC-treated water afterall, contains small amounts of DEPC. Although they generally should not affect the PCR process, it's better to use milliQ water as it's filtered, UV-ed and deionized (if I'm not mistaken). |
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| depc , mili , pcr , water |
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