I need immediate help!!
How to PCR primer designing?
I know primer design based on comparison of sequences of specific gene from GenBank.
Conserved regions were identified by using software Clustal X/W. Than primer A was designed based on the conserved amino acid sequence motif DD(T/I)(I/Y/F)D(A/V)Y(A/G). The sequence of sense primer A was 5’-GA(C/T) GA(C/T) III T(T/A)(T/C) GA(C/T) GCI (C/T)A(C/T) GG-3’.
My question is how to write the sequence of sense primer A from conserved amino acid sequence motif primer A ?? Please give references, concept and web site for further reading… you help I needed.