Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum
Register Search Today's Posts Mark Forums Read

PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


pcr problem for dna walking in genomic pcr

pcr problem for dna walking in genomic pcr - PCR - Polymerase Chain Reaction Forum

pcr problem for dna walking in genomic pcr - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-11-2009, 06:52 AM
Pipette Filler
Points: 387, Level: 7 Points: 387, Level: 7 Points: 387, Level: 7
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2009
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default pcr problem for dna walking in genomic pcr



i have some questions...im doing genomic pcr to design 3 sets of primer to be used for DNA walking Kit Seegene..

1. im using gradient pcr for every primer ive tried but the result was so weird...when i view my gel no band can be seen, also no smearing at all..why?

2. sometimes i got only pcr product with size 150bp only...how to optimize the mixture and parameter of the pcr to get good band?

3. any suggestion on how to do dna walking?

please help me...
Reply With Quote
Reply

Tags
dna , genomic , pcr , problem , walking


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
plasmid re-ligation problem Vagelis Protocols and Methods Forum 4 06-20-2012 08:29 PM
2D gel running problem cosmis85 Proteomics Forum 4 12-31-2009 05:26 AM
SDS-PAGE problem Steve Nothwehr Protocols and Methods Forum 2 10-16-2009 08:59 AM
2D gel running problem cosmis85 Protocols and Methods Forum 0 02-21-2007 10:08 AM
FW: SDS-PAGE problem Deanne Bell Protocols and Methods Forum 0 03-05-2004 05:27 PM


All times are GMT. The time now is 11:39 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11070 seconds with 16 queries