pcr problem for dna walking in genomic pcr
i have some questions...im doing genomic pcr to design 3 sets of primer to be used for DNA walking Kit Seegene..
1. im using gradient pcr for every primer ive tried but the result was so weird...when i view my gel no band can be seen, also no smearing at all..why?
2. sometimes i got only pcr product with size 150bp only...how to optimize the mixture and parameter of the pcr to get good band?
3. any suggestion on how to do dna walking?
please help me...
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