Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   PCR - Polymerase Chain Reaction Forum (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/)
-   -   pcr problem for dna walking in genomic pcr (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/70050-pcr-problem-dna-walking-genomic-pcr.html)

hazrina 08-11-2009 06:52 AM

pcr problem for dna walking in genomic pcr
 
i have some questions...im doing genomic pcr to design 3 sets of primer to be used for DNA walking Kit Seegene..

1. im using gradient pcr for every primer ive tried but the result was so weird...when i view my gel no band can be seen, also no smearing at all..why?

2. sometimes i got only pcr product with size 150bp only...how to optimize the mixture and parameter of the pcr to get good band?

3. any suggestion on how to do dna walking?

please help me...


All times are GMT. The time now is 11:18 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08989 seconds with 11 queries