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# Calculating DNA concentration in PCR product?

## Calculating DNA concentration in PCR product? - PCR - Polymerase Chain Reaction Forum

### Calculating DNA concentration in PCR product? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.

#1
07-19-2009, 10:20 PM
Pipette Filler

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Calculating DNA concentration in PCR product?

Calculating DNA concentration in PCR product?

A sample of DNA was isolated from a bacterial culture and the OD260 was determined using a spectrometer. In order to determine the OD260 reading the technical took 2 microliters of the sample and added 498 microliters of water. The OD260 was shown to be 0.70.

What is the dilution factor? The dilution factor is 1:250 (480+2=500, 2:500)

What is the concetration of the DNA in the sample? The concentration of the DNA in the sample is 8750 microliters/milileters.

The original tube contained 800 microliters. What is the total amount of DNA in the tube?
#2
07-21-2009, 11:48 AM
Post-Doc

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Re: Calculating DNA concentration in PCR product?

u can try [Only registered users see links. ] according to the calculator u have 8.75µg/µl of 800ul thus (8.75*800) = 7000µg

 Tags calculating , concentration , dna , pcr , product

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