| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
07-16-2009, 09:47 PM
| |||||||||||
| |||||||||||
 cDNA PCR troubleshoot I'm trying to do PCR using cDNA (confirmed to be of good quality w/ nanodrop and PCR with control primers - GAPDH). The primers I am trying this with are for the gene MMP14 and were designed using the NCBI primer designing tool. I inputted the refseq for the mRNA and used the primers it returned. when I do the PCR there are not even smears - only a collection of primers at the bottom. I've tried annealing temps from 50 to 60 degrees and nothing has worked. The product is 600 bp and i'm using taq hotstart. any advice would be appreciated! Last edited by bliu94; 07-16-2009 at 09:50 PM. Reason: n/a  |
|
| Tags |
| cdna , pcr , troubleshoot |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Help needed on aminoallyl-labeled cDNA | palver | Molecular Biology Articles and Protocols | 0 | 01-07-2009 07:40 AM |
| cDNA Synthesis and Amplification of POLY(A) mRNA by RT-PCR Protocol | domba | Real-Time PCR and Quantitative PCR Forum | 3 | 09-09-2008 02:48 PM |
| Commercial cDNA or cdna Synthesized Random hemer for Transfection | bhunni | DNA Techniques | 0 | 09-01-2007 07:12 AM |
| cdna | bhunni | DNA Techniques | 0 | 06-26-2007 08:45 AM |
| cDNA precipitation without coprecipitation of primers | Simone Marker | Protocols and Methods Forum | 1 | 03-05-2007 07:36 PM |
Linear Mode
