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Which two primer pairs would work to PCR amplify the double stranded DNA target...

Which two primer pairs would work to PCR amplify the double stranded DNA target... - PCR - Polymerase Chain Reaction Forum

Which two primer pairs would work to PCR amplify the double stranded DNA target... - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 07-15-2009, 12:18 AM
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Default Which two primer pairs would work to PCR amplify the double stranded DNA target...



...sequence shown below 3' .. GG? Which two primer pairs would work to PCR amplify the double stranded DNA target sequence shown below
3' .. GGCTGAGTGCTGGATGAGTGGGCTGAGCTTGCTA...5'
5' ...CCGACTCACGACCTACTCACCCGACTCGAACGAT...3'

Would it be...
5' GGCTGAGT 3' and 5' TACGAAGC 3'?
5' TGAGTCGG 3' and 5' CGAACGAT 3'?
5' CCGACTCA 3' and 5' ATCGTTCG 3'?
or
5' TACTCACC 3' and 5' GGTGAGTA 3'?
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