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-   -   Which two primer pairs would work to PCR amplify the double stranded DNA target... (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/69421-two-primer-pairs-would-work-pcr-amplify-double-stranded-dna-target.html)

Blaha 07-15-2009 12:18 AM

Which two primer pairs would work to PCR amplify the double stranded DNA target...
 
...sequence shown below 3' .. GG? Which two primer pairs would work to PCR amplify the double stranded DNA target sequence shown below
3' .. GGCTGAGTGCTGGATGAGTGGGCTGAGCTTGCTA...5'
5' ...CCGACTCACGACCTACTCACCCGACTCGAACGAT...3'

Would it be...
5' GGCTGAGT 3' and 5' TACGAAGC 3'?
5' TGAGTCGG 3' and 5' CGAACGAT 3'?
5' CCGACTCA 3' and 5' ATCGTTCG 3'?
or
5' TACTCACC 3' and 5' GGTGAGTA 3'?


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