| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
07-09-2009, 01:31 AM
| |||||||||||
| |||||||||||
 qPCR melting curve trouble Dear everyone  I'm new for qPCR only several monthes. And using the PCR production as SYBR Green qPCR's template to generate the standard curve. Normally we use the size below 300bp, but some of my productions have to be around 550pb, is it acceptable? And there are two peaks of the melting curve that quite near to each other. How can deal with it? (I already modified the annealling temperature and primer concentration but useless.  )And another melting curve is very broad but single peak, I'm afraid there are two peaks inside. So maybe it should with the same problem as before? Sorry for poor English. Would you help me please?  Last edited by amoon_sasa; 07-13-2009 at 05:49 AM.  |
|
| Tags |
| curve , melting , melting curves , qpcr , sybr green , trouble |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| qPCR NEWS August 2009 - focus on HRM | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 08-11-2009 02:12 PM |
| qPCR NEWS December 2008 | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 12-17-2008 08:03 AM |
| qPCR Newsletter July 2008 - main focus on qPCR optimisation | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 07-28-2008 02:48 PM |
| qPCR NEWSLETTER - September 2007 - qPCR 2007 talks online | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 09-25-2007 12:34 PM |
| qPCR 2007 - CALL for scientific contributions | Editor www.Gene-Quantification.info | Protocols and Methods Forum | 0 | 01-22-2007 01:24 PM |
Re: qPCR melting curve trouble 
Linear Mode
