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06-29-2009, 05:30 PM
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 Selecting Baseline Thresholds, digital pcr Hi, I am observing a reactions fluorescence signal after 50 cycles (observing hundreds of reactions simultaenously). Only a few reactions contain template molecules. The result is most of the reactions have a given intensity level (low-no amplification since there are no molecules present), few reactions with high intensity (corresponding to amplified target molecules) and some very annoying reactions showing a medium intensity level (most likely noise, but difficult to distinguish from amplified reactions in some cases). Can anyone suggest how I could apply a baseline threshold and determine whether amplified product is present for a given reaction? A link to literature on the topic would be great even, I cant seem to find anything on web, Thanks, Mark  |
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| digital pcr , pcr prototyping , threshold |
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