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PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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RT-PCR semiquantitative

RT-PCR semiquantitative - PCR - Polymerase Chain Reaction Forum

RT-PCR semiquantitative - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 06-19-2009, 06:26 PM
Pipette Filler
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Question RT-PCR semiquantitative



Hello,
I have to do semi-quantitative PCR in order to study the regulation of
expression of some genes. I’d like to know what to do if I can’t detect my genes of interest, even when GADPH has already reached the plateau phase.
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