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primers & +ve control

primers & +ve control - PCR - Polymerase Chain Reaction Forum

primers & +ve control - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 04-14-2009, 02:38 PM
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Default primers & +ve control



Hi,

I have strong bands in my positve control but no bands in my samples. What that means? What goes wrong? (expression of the gene should occur in these samples tho).

Thank u
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Old 04-18-2009, 04:07 PM
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Default Re: primers & +ve control

If you have strong bands in your control samples you probably shouldn't be worried so much about reaction conditions because this seems to be ok.
Try to interpret the data in different ways.
Should the expression of the gene really occur in those samples?
Is the method you use sensitive enough to visualise the bands if the expression occurs?
Were the samples prepared in the same way as your positive control?
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