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primers & +ve control
I have strong bands in my positve control but no bands in my samples. What that means? What goes wrong? (expression of the gene should occur in these samples tho).
Re: primers & +ve control
If you have strong bands in your control samples you probably shouldn't be worried so much about reaction conditions because this seems to be ok.
Try to interpret the data in different ways.
Should the expression of the gene really occur in those samples?
Is the method you use sensitive enough to visualise the bands if the expression occurs?
Were the samples prepared in the same way as your positive control?
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