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Standard curve in RT-PCR

Standard curve in RT-PCR - PCR - Polymerase Chain Reaction Forum

Standard curve in RT-PCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #11  
Old 05-09-2009, 09:05 PM
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Default Re: Standard curve in RT-PCR

I've just sent you the answer. I hope I helped you a bit. Good luck
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Old 01-13-2010, 07:39 AM
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Default Re: Standard curve in RT-PCR

Hi
Here I am new in the use of realtime pcr for analysis of genes after heat shock treatment. I will wish to ask a basic question to understand as I proceed. Is it possible to scale down the primer optimisation protocol suggested by the manufacturer? Secondly, I have designed primers for my experiments and use one to test if gDNA is present(primer spanning exon intron boundary) in a nested reaction. Do I need to design outer primers for this nest?
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