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PCR contamination HELP!

PCR contamination HELP! - PCR - Polymerase Chain Reaction Forum

PCR contamination HELP! - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 03-13-2009, 07:56 AM
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Unhappy PCR contamination HELP!



I have faced the contamination problem in my PCR negative control. I have changed all the reagents and primers, used autoclaved distilled water to prepare mixture, but the band still there in the negative control. I really cant think out where is going wrong. And, the band in the negative control is always the same size with the sample band (~300bp) but more faint than the sample band.
Anyone can tell me what is happening?Thanks.
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Old 03-13-2009, 08:16 AM
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Default Re: PCR contamination HELP!

Did you change the pipetters and tips?
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Old 03-13-2009, 04:05 PM
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Default Re: PCR contamination HELP!

Your problem sounds similar to another poster. Please look at my reply to him/her:

[Only registered users see links. ]
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The Following User Says Thank You to jonoave For This Useful Post:
admin (04-06-2009)
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