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- - PCR contamination HELP! (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/65152-pcr-contamination-help.html)

eevian

03-13-2009 07:56 AM

PCR contamination HELP!

I have faced the contamination problem in my PCR negative control. I have changed all the reagents and primers, used autoclaved distilled water to prepare mixture, but the band still there in the negative control. I really cant think out where is going wrong. And, the band in the negative control is always the same size with the sample band (~300bp) but more faint than the sample band.
Anyone can tell me what is happening?Thanks.

alexseagon

03-13-2009 08:16 AM

Re: PCR contamination HELP!

Did you change the pipetters and tips?

jonoave

03-13-2009 04:05 PM

Re: PCR contamination HELP!

Your problem sounds similar to another poster. Please look at my reply to him/her:

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