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-   -   PCR contamination HELP! (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/65152-pcr-contamination-help.html)

eevian 03-13-2009 07:56 AM

PCR contamination HELP!
 
I have faced the contamination problem in my PCR negative control. I have changed all the reagents and primers, used autoclaved distilled water to prepare mixture, but the band still there in the negative control. I really cant think out where is going wrong. And, the band in the negative control is always the same size with the sample band (~300bp) but more faint than the sample band.
Anyone can tell me what is happening?Thanks.

alexseagon 03-13-2009 08:16 AM

Re: PCR contamination HELP!
 
Did you change the pipetters and tips?

jonoave 03-13-2009 04:05 PM

Re: PCR contamination HELP!
 
Your problem sounds similar to another poster. Please look at my reply to him/her:

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