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primer concentration

primer concentration - PCR - Polymerase Chain Reaction Forum

primer concentration - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #1  
Old 01-11-2009, 12:05 PM
Pipette Filler
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Default primer concentration



hi there
it's a little bit stupid question but I have to make sure..
about primer concentration:
I have 30nM of lyophilized primer, I add 300 ul water or TE buffer and get 100uM/L stock solution. If I add 600 ul water I get 200uM/L stock solution???
So to prepare 10uM/L work solution I have to take 20ul primer stock solution and add 80 ul water?? Am I right? Question is cause I did the dilution one more time after did it before
thank You in advance!!!
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Old 01-20-2009, 08:52 AM
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Default Re: primer concentration

Hi Ineska welcome,
for primer amount check out this page:
[Only registered users see links. ]

Also, it doesn't matter too much how you store your primers, the important thing is the final solution (concentration).

What is your final concentration of primers?
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