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problem in amplification after miniprep

problem in amplification after miniprep - PCR - Polymerase Chain Reaction Forum

problem in amplification after miniprep - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 12-20-2008, 01:34 PM
Pipette Filler
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Unhappy problem in amplification after miniprep



hi all ,
i usually grow colonies by ChEST library and do colony PCR , till that time amplification of my gene occurs but when i do miniprep of those colonies and then set a amplification reaction , no amplification happens . i have done it several times but results are same . please tell me some clues what thing i am doing wrong .
thank you

Regards

Sandeep
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