| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| HI, i've been trying to get expression of certain genes after stimulating cells with TNF-alpha, but in my PCR i'm only getting the beta actin control band.My supervisor thinks the TNF may not be working, how would i test for activity of TNF before stimulating the cells with it? |
|
#2
| ||||||||||||
| ||||||||||||
| Quote:
|
| Tags |
| gene , interest , pcr , product |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| problem with TOPO TA cloning.... | nusratali610 | Molecular Cloning Forum | 34 | 05-08-2013 09:28 PM |
| Product detectible on gel, melting curve peak, but no amplification curve | emucaki | Real-Time PCR and Quantitative PCR Forum | 4 | 08-24-2009 08:54 PM |
| GSTP1 promotor - unspecific product | rob86 | PCR - Polymerase Chain Reaction Forum | 1 | 07-29-2008 06:58 AM |
| Please help: A proteolyzed product running bigger on an SDS gel? | cv2135@columbia.edu | Protocols and Methods Forum | 0 | 05-29-2008 05:19 AM |