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No PCR product for gene os interest

No PCR product for gene os interest - PCR - Polymerase Chain Reaction Forum

No PCR product for gene os interest - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 09-24-2008, 07:49 AM
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Question No PCR product for gene os interest



HI, i've been trying to get expression of certain genes after stimulating cells with TNF-alpha, but in my PCR i'm only getting the beta actin control band.My supervisor thinks the TNF may not be working, how would i test for activity of TNF before stimulating the cells with it?
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Old 09-24-2008, 02:10 PM
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Default Re: No PCR product for gene os interest

Quote:
Originally Posted by Grainne View Post
HI, i've been trying to get expression of certain genes after stimulating cells with TNF-alpha, but in my PCR i'm only getting the beta actin control band.My supervisor thinks the TNF may not be working, how would i test for activity of TNF before stimulating the cells with it?
look for the activation of NF-kB in your treated cells versus your non-treated control cells.
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