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#1
09-19-2008, 12:04 AM
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 PCR with Oligo Primers Larger than 30-mers? Hello, i want to use oligonucleotide primers larger than 30-mers ie 45 nucleotide oligonucleotides. This is because i would like to add the restriction site plus a few bp on either side of the cleavage site to make efficient restriction digestion after PCR and cloning is this ok? how should i optimise my sequence? thanks again  |
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| 30mers , larger , oligo , pcr , primers |
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