|Register||Search||Today's Posts||Mark Forums Read|
|PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.|
| ||LinkBack||Thread Tools||Display Modes|
PCR duplex bad problem
I’m tring to reproduce a ASO-PCR able to discriminate between two different strains. The ASO is due by using three different primer: a forward one and two reverse pair; the former reverse is able, with the forward, to amplify an internal control, while the latter reverse gives an amplicon shorter than the previously one but able to discriminate between the two different strains. So the discrimination power of the PCR is due only by the second reverse primer. The problem is these strain differ from each other from only two pair of bases. I tried a lot of experiments but so far I was not able to discriminate, I mean, the two PCR are positive with the same sample, so I can’t cluster my samples. Does somebody know a good site having informations and troubleshooting about ASO-PCR?
Many thanks in advances.
Last edited by amicia; 09-16-2008 at 06:34 AM.
|bad , duplex , pcr , problem|
|Thread||Thread Starter||Forum||Replies||Last Post|
|2D PAGE problem||antheab||Peptide Forum||3||12-26-2010 03:28 PM|
|SDS-PAGE problem||Steve Nothwehr||Protocols and Methods Forum||2||10-16-2009 08:59 AM|
|weird problem in transfecting 293 cells in 24-well plate||ÕÔÇï»Ô||Cell Biology and Cell Culture||1||04-17-2007 03:54 AM|
|N-body problem||Torstein Raanes||Physics Forum||1||12-01-2003 09:31 AM|
|Integration Problem / Finding Contour lines Problemfirstname.lastname@example.org||Physics Forum||2||09-07-2003 11:28 PM|