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How to use Multiplex PCR?

How to use Multiplex PCR? - PCR - Polymerase Chain Reaction Forum

How to use Multiplex PCR? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 02-20-2008, 10:04 AM
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Exclamation How to use Multiplex PCR?



how we can use multiplex PCR
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Old 01-28-2013, 09:48 AM
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Default Re: How to use Multiplex PCR?

[Only registered users see links. ]Single Template PCR Reaction -This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template.
Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube. Presence of multiple primers- may lead to cross hybridization with each other and the possibility of mis-priming with other templates.
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