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#2
01-28-2013, 08:48 AM
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 Re: How to use Multiplex PCR? [Only registered users see links. ]Single Template PCR Reaction -This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template. Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube. Presence of multiple primers- may lead to cross hybridization with each other and the possibility of mis-priming with other templates.  |
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How to use Multiplex PCR? 
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