How to use Multiplex PCR?
how we can use multiplex PCR
Re: How to use Multiplex PCR?
[Only registered and activated users can see links. Click Here To Register...]Single Template PCR Reaction -This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template.
Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube. Presence of multiple primers- may lead to cross hybridization with each other and the possibility of mis-priming with other templates.
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