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-   -   How to use Multiplex PCR? (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/3536-how-use-multiplex-pcr.html)

padma 02-20-2008 10:04 AM

How to use Multiplex PCR?
 
how we can use multiplex PCR

davidflora 01-28-2013 09:48 AM

Re: How to use Multiplex PCR?
 
[Only registered and activated users can see links. Click Here To Register...]Single Template PCR Reaction -This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template.
Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube. Presence of multiple primers- may lead to cross hybridization with each other and the possibility of mis-priming with other templates.


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