How to use Multiplex PCR?
 

how we can use multiplex PCR

Re: How to use Multiplex PCR?
 

[Only registered and activated users can see links. Click Here To Register...]Single Template PCR Reaction -This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template.
Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube. Presence of multiple primers- may lead to cross hybridization with each other and the possibility of mis-priming with other templates.