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PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.

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PCR - Polymerase Chain Reaction Forum


PCR contamination

PCR - Polymerase Chain Reaction Forum

PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.



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  #1 (permalink)  
Old 06-29-2008, 09:31 AM
Pipette Filler
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Default PCR contamination

I have a problem during PCR program. add templete DNA, dNTP, primer,

polymerase, etc..to ep tube. then I forgot to add oiling U-U

so.. I am nervous for fear of contamination.

Does it matter of this situation? If you shoud be clear up this problem, I will be rearlly preciative of your comment.^_^
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  #2 (permalink)  
Old 07-02-2008, 01:06 PM
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Default Re: PCR contamination

Hello Doohe,

welcome to the forum.

Its ok Doohe I am sure you should be ok. Always try things thats how everyone learns. Let us know how the PCR goes and post a gel picture and we can take a look and help.

When you say you forgot to add oiling U-U do you mean you forgot to add oil to the PCR machine?

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Old 08-05-2008, 04:38 PM
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Default Re: PCR contamination

There shouldn't be a problem with contamination. I think that the oil is more to prevent the liquid in the PCR tube from evaporating when the solution is being heated. If your PCR machine can set up a heated lid while running, that might also prevent evaporation.
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