PCR contamination

[Doohe*]

I have a problem during PCR program. add templete DNA, dNTP, primer,

polymerase, etc..to ep tube. then I forgot to add oiling U-U

so.. I am nervous for fear of contamination.

Does it matter of this situation? If you shoud be clear up this problem, I will be rearlly preciative of your comment.^_^

[admin]

Hello Doohe,

welcome to the forum.

Its ok Doohe I am sure you should be ok. Always try things thats how everyone learns. Let us know how the PCR goes and post a gel picture and we can take a look and help.

When you say you forgot to add oiling U-U do you mean you forgot to add oil to the PCR machine?

[keithbrent2000]

There shouldn't be a problem with contamination. I think that the oil is more to prevent the liquid in the PCR tube from evaporating when the solution is being heated. If your PCR machine can set up a heated lid while running, that might also prevent evaporation.