| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
 pcr Videos | ||
| ||
| PCR - Polymerase Chain Reaction Forum | |||||
|
|
| |||
 |
 |
| | LinkBack | Thread Tools | Display Modes |
06-27-2008, 01:54 PM
| |||||
| |||||
 PCR, incomplete products? Hello, I am using iproof high-fedility polymerase to amplify a DNA fragment 6kb in size from a genome. With Taq that's almost impossible but iproof is supposed to go through it with ease. I do get the band but I also get alot of incomplete products in the form of smaller and lighter bands. Could they be single stranded DNA moelcules resulting from the polymerase jumping off before it completes replication? I uploaded the gel. it's the first lane after the ladder. Does MgCl2 have anything to do with it? Any help is good. Thanks        |
| Today
| ||||
| ||||
 Sponsored Links |
|
| Thread Tools | |
| Display Modes | |
|
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Agarose gel electrophoresis of PCR products - Method and Protocol | domba | PCR - Polymerase Chain Reaction Forum | 4 | 05-23-2008 04:42 PM |
| How do You Purifiy Your PCR Products? | proatpcr | PCR - Polymerase Chain Reaction Forum | 1 | 10-27-2007 03:46 AM |
| Short Unspecific PCR Products | yuoian | PCR - Polymerase Chain Reaction Forum | 1 | 09-05-2007 03:11 PM |
| pcr amplified products | lakshb | PCR - Polymerase Chain Reaction Forum | 1 | 07-30-2007 04:48 AM |
Linear Mode
