Originally Posted by bhaktamehul
i want to know what are the causes of pcr smear. coz i have been having some smear from last one week , but early it workd great... i even tried new dna samples, made changes in annealing temp and time and also used same primer from other lab(where it is working),,
so i just want to know what can be the causes of smearing , so that i can sort out problem...
The first reason for smear is DNA which was degradation , must prepare new DNA fresh with good concentration and try again .
cheak the primer on the agarose gel