Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > PCR - Polymerase Chain Reaction Forum
Register Search Today's Posts Mark Forums Read

PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Nanodrop

Nanodrop - PCR - Polymerase Chain Reaction Forum

Nanodrop - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-12-2008, 03:51 PM
Pipette Filler
Points: 594, Level: 11 Points: 594, Level: 11 Points: 594, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Nanodrop



Hello all
Is there anyone out there who uses Genecelan III kit for PCR product purification? If so, could you quantify that using Nanodrop?
Thanks
Reply With Quote
  #2  
Old 04-14-2008, 04:44 PM
Graduate Student
Points: 2,923, Level: 35 Points: 2,923, Level: 35 Points: 2,923, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2006
Location: Ithaca, NY
Posts: 145
Thanks: 0
Thanked 17 Times in 12 Posts
Default Re: Nanodrop

I don't use Geneclean but have used Invitrogen and Qiagen kits for PCR product purification. I tend to get on the order of 50 ng/uL, which is easily in the Nanodrop's measuring range of 2-3700 ng/uL for dsDNA.
Reply With Quote
  #3  
Old 05-02-2008, 03:23 PM
Pipette Filler
Points: 594, Level: 11 Points: 594, Level: 11 Points: 594, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Nanodrop

thanks
Reply With Quote
Reply

Tags
nanodrop


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Tell me about using a Nanodrop for protein quantitation Bonnie Protein Science 5 06-18-2010 10:46 AM
RNA and Nanodrop Arastho RNA Techniques Forum 2 06-18-2010 10:43 AM
PCR quantification on Nanodrop? niki_nje PCR - Polymerase Chain Reaction Forum 10 09-18-2009 09:33 AM
NanoDrop ND-1000 spectrophotometer Guenter Jost Protocols and Methods Forum 3 11-19-2003 06:29 PM


All times are GMT. The time now is 05:45 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11833 seconds with 16 queries