Rt Pcr

Kanwal · #1 (**permalink**) 04-11-2008, 06:53 AM

After doing PCR i got very light band of IL-3.Plz tell me what should i do.should i increase the concentarion of cDNA?or any other suggestion?

danfive · #2 (**permalink**) 04-11-2008, 05:51 PM

You can increase the RNA template, cDNA template (if 2 step RT-PCR), PCR cycles.
If you have less than 40 thermocycles, begin there. If you already do 40 or more cyles, begin with either RNA or cDNA whichever is most convenient.

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Rt Pcr

PCR - Polymerase Chain Reaction Forum

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