I'm getting very tiny biopsy samples for my PCR analysis. I tried to purify DNA from these samples using conventional methods, adding glycogen, etc, but never got any detectable DNA to work with. I ended up incubating my samples in lysis buffer @95C for 10min, spinning them down, and using supernatant to run PCR. Sometimes this method works and I get PCR products, sometimes it does not. My guess is that I have contaminant(s) that inhibits PCR.
Does anybody have any good suggestions on how to clean up my samples?