Originally Posted by dgadiyar
Kindly let me know how to design an internal amplification control for detection of hil A gene of Salmonella in stool samples. this is mainly to rule out PCR inhibitors in the DNA extracted from stool
thanks and regards
one does not have to reinvent the wheel to get an internal control
The best way to do this is to find any article which shows a good primer set for the detection of the human beta actin gene. The human beta actin fragment should coamplify with your Salmonella genes.
for Amicia: the problem with plasmids is that because of their circularity, and copy number, this form of DNA can withstand more abuse than chromosomal DNA and these are detected much more easily than the original DNA target presumptively of chromosomal origin.