| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
 pcr Videos | |||||
· · · rt-pcr-part-1 Video |
· · · real-time-pcr-protocol-of-gm-soybean-dna Video |
· · · pcr-polymerase-chain-reaction Video | |||
| PCR - Polymerase Chain Reaction Forum | |||||
|
|
| |||
 |
 |
| | LinkBack | Thread Tools | Display Modes |
01-24-2008, 04:57 AM
| |||||
| |||||
 Internal Amplification control in PCR Kindly let me know how to design an internal amplification control for detection of hil A gene of Salmonella in stool samples. this is mainly to rule out PCR inhibitors in the DNA extracted from stool thanks and regards dgadiyar        |
| Today
| ||||
| ||||
 Sponsored Links |
|
01-24-2008, 04:44 PM
| ||||||
| ||||||
 Re: Internal Amplification control in PCR Spiked controls can be used to rule out PCR inhibitors. If you make a set of PCR reactions and add sample DNA template then spike with your positive control, you should get amplification in all reaction tubes, unless PCR inhibitors are present. |
|
| Tags |
| pcr, polymerase chain reaction |
| Thread Tools | |
| Display Modes | |
|
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| internal control for plant proteins in WB | izacil | Western Blot Forum | 1 | 04-09-2008 02:21 PM |
| GADPH Loading Control Western Blot Problem | jrscientist | Western Blot Forum | 1 | 02-07-2008 09:59 AM |
| Loading Control | montMolec | Western Blot Forum | 4 | 10-21-2007 05:49 PM |
| No PFU PCR Amplification! help! why? | ale | PCR - Polymerase Chain Reaction Forum | 5 | 09-14-2007 04:39 PM |
| Peering Into The Shadow World Of RNA: Crosstalk May Control The Genome | kiki06 | General Science Questions and Layperson Board | 1 | 12-06-2006 10:51 AM |
Linear Mode
