common Polymerases

ete · #1 (**permalink**) 01-15-2008, 01:04 PM

Hi!

I'm implementing a program, which calculates the annealing temperature of an oligo (4 different methods). Since you can use it for PCR reactions, I want to give a choice of common Polymerases (Taq, Proof Reading, Pfu) to put automatically the right salt concentrations.

Since I use most of the time Qiagen Taq Master Mix and Invitrogen's Thermal Ace, I would like to know what is used in other labs.

Some feedback would be nice

Stefanie

admin · #2 (**permalink**) 07-04-2008, 10:26 AM

Hi here is a good guide on polymerases:
http://www.pcrstation.com/pcr-polymerases/

However for specific polymerases I use Promegas Taq as its cheap and pretty good. For error and proof reading I would use a better version such as a Gold or proof reading one.

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common Polymerases

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