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02-12-2006, 06:53 PM
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 PCR of long DNA fragments I have to do an extended PCR to clone a large DNA fragment. I have tried the more processive Taq enzymes on sale to no avail. I cannot get my band of length 5 kb. I have a feeling that it is the cycling program. Any one have any ideas on how to improve long pcrs? thanks        |
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09-26-2008, 03:23 PM
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 Re: PCR of long DNA fragments PCR donīt are able to copy from a determine size up but i donīt know wich size is this... wath i do when i have to clone a large fragment is to divide it in smaller fragments... i use primers insted of using 2 primers i use 4 or 6, etc... i hope that hellp... |
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| dna , fragments , long , pcr |
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