|Register||Search||Today's Posts||Mark Forums Read|
|PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.|
| ||LinkBack||Thread Tools||Display Modes|
. On which side of the targeted sequence on a strand of DNA (coding or
non-coding) must a PCR primer bind? I just need the answer
Re: . On which side of the targeted sequence on a strand of DNA (coding or
i hope i understand ur question correctly. hmtasultan, u are asking whether DNA binds on the positive strand (5'-3') or negative strand (3'-5')? Well general answer would be pcr primer bind to the strand that contain sequences complimentary to the primer sequences.
maybe u can rephrase the question so we all can understand the reason for ur question. are u designing primer? remember, DNA polymerase extend 3' end.
|coding , dna , sequence , side , strand , targeted|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Human Cytome Project - Update 24 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||1||08-01-2010 02:18 PM|
|effexor side effects sweating zocor effects on gallbladder sideeffects expired firstname.lastname@example.org||Cell Biology and Cell Culture||0||03-27-2008 12:14 PM|
|A Human Cytome Project - an idea - Update 14 March 2005||Peter Van Osta||Cell Biology and Cell Culture||0||03-14-2005 01:27 PM|
|Human Cytome Project - Update 6 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||0||01-06-2005 10:18 AM|