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| PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory. |
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| non-coding) must a PCR primer bind? I just need the answer |
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| i hope i understand ur question correctly. hmtasultan, u are asking whether DNA binds on the positive strand (5'-3') or negative strand (3'-5')? Well general answer would be pcr primer bind to the strand that contain sequences complimentary to the primer sequences. maybe u can rephrase the question so we all can understand the reason for ur question. are u designing primer? remember, DNA polymerase extend 3' end. |
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| coding , dna , sequence , side , strand , targeted |
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