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. On which side of the targeted sequence on a strand of DNA (coding or

. On which side of the targeted sequence on a strand of DNA (coding or - PCR - Polymerase Chain Reaction Forum

. On which side of the targeted sequence on a strand of DNA (coding or - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-31-2008, 10:16 PM
Pipette Filler
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Default . On which side of the targeted sequence on a strand of DNA (coding or



non-coding) must a PCR primer bind? I just need the answer
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Old 12-03-2008, 03:38 PM
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Default Re: . On which side of the targeted sequence on a strand of DNA (coding or

i hope i understand ur question correctly. hmtasultan, u are asking whether DNA binds on the positive strand (5'-3') or negative strand (3'-5')? Well general answer would be pcr primer bind to the strand that contain sequences complimentary to the primer sequences.

maybe u can rephrase the question so we all can understand the reason for ur question. are u designing primer? remember, DNA polymerase extend 3' end.
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