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If the primers in a PCR reaction were the same for multiple studentís samples...?

If the primers in a PCR reaction were the same for multiple studentís samples...? - PCR - Polymerase Chain Reaction Forum

If the primers in a PCR reaction were the same for multiple studentís samples...? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-30-2008, 12:19 PM
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Default If the primers in a PCR reaction were the same for multiple studentís samples...?



Would there be any difference in the nucleotide sequence for the PCR amplified band on different student lanes?
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Old 12-03-2008, 03:42 PM
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Default Re: If the primers in a PCR reaction were the same for multiple studentís samples...?

if sample, mastermix, no contamination and primer is specific generally there is no difference. However I have seen different ppl running the same pcr yet one of them get positive result the other negative. By the way do you notice any different in gel visualization from pcr done by different student?
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Old 12-03-2008, 09:53 PM
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Default Re: If the primers in a PCR reaction were the same for multiple studentís samples...?

I've seen that and I guess it must have been some kind of contamination (one had unspecific band besides the specific one) - never traced down the source of that particular contamination.
Huge differences occur with real-time PCR but usually not in products but in amounts of DNA and reagents added.
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