If the primers in a PCR reaction were the same for multiple student’s samples...?

Ms. Maria Fun School · #1 10-30-2008, 12:19 PM

Would there be any difference in the nucleotide sequence for the PCR amplified band on different student lanes?

butters · #2 12-03-2008, 02:42 PM

if sample, mastermix, no contamination and primer is specific generally there is no difference. However I have seen different ppl running the same pcr yet one of them get positive result the other negative. By the way do you notice any different in gel visualization from pcr done by different student?

Aga · #3 12-03-2008, 08:53 PM

I've seen that and I guess it must have been some kind of contamination (one had unspecific band besides the specific one) - never traced down the source of that particular contamination.
Huge differences occur with real-time PCR but usually not in products but in amounts of DNA and reagents added.

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If the primers in a PCR reaction were the same for multiple student’s samples...?

If the primers in a PCR reaction were the same for multiple student’s samples...? - PCR - Polymerase Chain Reaction Forum

If the primers in a PCR reaction were the same for multiple student’s samples...? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.