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11-28-2007, 10:02 PM
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 long-range PCR polymerases? I am looking to do some long-range PCRs (5-30kB) of mouse genomic DNA and am wondering if anyone has any suggestions on the most robust and cost-efficient brand of long-range PCR? I have heard that Roche's "Expand" works well, but want to see if there are other suggestions out there... Or do I even need to buy a special kit? Perhaps as long as there's some proofreading enzyme in there and I alter the cycling conditions, I'll get good results?? Thanks!        |
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