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PCR linear DNA single nucleotide mutation

PCR linear DNA single nucleotide mutation - PCR - Polymerase Chain Reaction Forum

PCR linear DNA single nucleotide mutation - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-21-2008, 03:21 PM
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Default PCR linear DNA single nucleotide mutation



hi all,

I have a question about linear DNA single nucleotide mutation. I have a sequence of GFP. I want to add an NcoRI site in front of the first base so it could conjugate with another promoter I have (which has a NcoRI site at the end). But the GFP I have right now has a internal NcoRI site. I want to mutate it so it is clean inside.

What method do you guys think is the best one to do the mutation? Overlapping PCR maybe? I am eager to learn things from you all. Smile

Cheers
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dna , linear , mutation , nucleotide , pcr , single


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