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11-09-2007, 02:04 PM
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 How to get rid of PCR primer dimers Hi!! I am going to post some methods of how to get rid of PCR primer dimers as this seems to be a common question.  1) Set the PCR at a different annealing temperature ranging from 60 C to 72 degrees (usually a higher temperature). The best method is to actually use a gradient to assess for the least primer dimers. 2) Also you should try titrating the concentration of Mg2+ from 1.5 to 2.5 mM. 3) Decrease the primer concentration (you may be using too much!).        |
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