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How to get rid of PCR primer dimers

How to get rid of PCR primer dimers - PCR - Polymerase Chain Reaction Forum

How to get rid of PCR primer dimers - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 11-09-2007, 03:04 PM
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Smile How to get rid of PCR primer dimers



Hi!!
I am going to post some methods of how to get rid of PCR primer dimers as this seems to be a common question.


1) Set the PCR at a different annealing temperature ranging from 60 C to 72 degrees (usually a higher temperature). The best method is to actually use a gradient to assess for the least primer dimers.

2) Also you should try titrating the concentration of Mg2+ from 1.5 to 2.5 mM.

3) Decrease the primer concentration (you may be using too much!).
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