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What happens when I get lotsof different bands in my samples when my control works ok

What happens when I get lotsof different bands in my samples when my control works ok - PCR - Polymerase Chain Reaction Forum

What happens when I get lotsof different bands in my samples when my control works ok - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 11-08-2007, 10:28 AM
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Exclamation What happens when I get lotsof different bands in my samples when my control works ok



Hi everyone,
it has been quite a long time now that i am facing a problem with my PCRs that drives me crazy
My control sample works perfect and gives me a nice sharp band. However, in my samples in which I want to see if there is or not the gene I am looking for i get lots of different bands which sometimes appear and sometimes not.

Any suggestions of what that means??? except that i shall stop doing PCRs?
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Old 11-08-2007, 05:06 PM
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Default Re: What happens when I get lotsof different bands in my samples when my control work

Maybe toss some extraction reagents that could be contaminated. And work on getting better sample preparation consider doing DNAse/RNase digestion.
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