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11-08-2007, 09:28 AM
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 What happens when I get lotsof different bands in my samples when my control works ok Hi everyone, it has been quite a long time now that i am facing a problem with my PCRs that drives me crazy  My control sample works perfect and gives me a nice sharp band. However, in my samples in which I want to see if there is or not the gene I am looking for i get lots of different bands which sometimes appear and sometimes not.  Any suggestions of what that means??? except that i shall stop doing PCRs?         |
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11-08-2007, 04:06 PM
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 Re: What happens when I get lotsof different bands in my samples when my control work Maybe toss some extraction reagents that could be contaminated. And work on getting better sample preparation consider doing DNAse/RNase digestion. |
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