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PCR - Polymerase Chain Reaction Forum


Faint PCR Product

PCR - Polymerase Chain Reaction Forum

PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.



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Old 11-06-2007, 12:39 PM
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Question Faint PCR Product

Hello, I have a faint pcr product (the right one finally!). I ran the agarose gel and the band is faint even after optimization and many cycles.

Are there any tips to get this faint band more stronger?

thanku all who help !
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Old 11-06-2007, 02:51 PM
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Default Re: Faint PCR Product

If you have any PCR reaction left use it as template for a repeat PCR; or use more DNA template/Primers/dNTPs in PCR reaction.
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