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Agarose gel electrophoresis of PCR products - Method and Protocol

Agarose gel electrophoresis of PCR products - Method and Protocol - PCR - Polymerase Chain Reaction Forum

Agarose gel electrophoresis of PCR products - Method and Protocol - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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  #11  
Old 12-10-2011, 05:24 AM
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Default Re: Agarose gel electrophoresis of PCR products - Method and Protocol

you are talking about very important details
mahmoud manama
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Old 02-01-2012, 10:55 AM
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Default Re: Agarose gel electrophoresis of PCR products - Method and Protocol

Thanks a lot for the informative protocol. Now how can I prepare Ethidium bromide to be used as an Intercalating dye for visual detection under UV straight from the tubes. What is the best concentration?
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  #13  
Old 02-24-2012, 03:39 AM
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Default Re: Agarose gel electrophoresis of PCR products - Method and Protocol

2ul to 4 ul of 10ug/ul ethidium bromide stock per 100ml gel is very good.
the size of loading product have affect
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